The TissueFAXS system not only allows for the delineation of tumour regions, non-tumour regions, but even stratification to differentiate specific tissue ranges. On the basis of identifying the tumour epithelium of colorectal cancer samples, the system divides the mesenchyme into different layers according to the distance relationship between mesenchyme and tumour. The distribution pattern of Treg cells in the mesenchyme and the correlation between their functional status and distribution are then counted. The further refinement of the tumour microenvironment analysis was achieved.

Specific neuronal quantitative analysis solutions, using morphological identification methods, can identify neuronal cell bodies and protrusions in many different sample types (HE, IHC, IF, etc.). The size, length, and number of branches of cell bodies and protrusions are identified and quantified in immunohistochemical samples according to the differences in neuronal appearance between different functions and regions, and unipolar/multi-level neurons are distinguished according to different functions and regions. It is also possible to identify and quantify the number of contacts between different neuronal protrusions, by using the spatial location information of neuronal cell bodies and nerve protrusions.

The use of multicolour technology to mark different lymphocytes is a common method to study the immune status of the tumour microenvironment. With TissueFAXS, we can quantify the nuclear, plasma and membrane colouration of each cell in tissue sections, and use scatter plots to link with the original images to determine the co-expression of multiple markers and easily classify the cell phenotype.

Using specific nucleic acid fluorescent probes and immunofluorescence technology to label relevant proteins and nucleic acids, we can not only accurately quantify the number and location of viruses in and out of the cell from the images, but also establish a standard for quantifying viral loads that can be judged by the naked eye, and at the same time analyse the process of viral invasion and the spatial relationship between the virus proliferation and the tissues!