TissueFAXS UPRIGHT

The device is based on tissue in situ class flow analysis technology, for single cell quantitative analysis experiments, usually choose flow cytometry as the experimental method. Although it can do accurate single-cell quantification, for quantitative analysis of tissue sections, it can only rely on the image quantification of staining, but the location information of tissue in situ is missing; and the traditional image quantification based on the staining of sections, the accuracy of its identification and the precision of its analysis can not meet the increasingly in-depth scientific research needs.

The TissueFAXS system combines the flow scatter plot analysis technology with the in-situ information of the image, which not only obtains accurate single-cell level quantitative data, but also combines in-depth location information of the tissue in-situ, which can be used to quantify the tissue structure, sieve and quantify the tissue area, and the distribution of the target proteins or target cells in tissues, etc., which not only provides more comprehensive analysis data, but also offers new solution ideas and research ideas for the future research. It not only provides more comprehensive analysis data, but also puts forward new solution ideas and research directions for future research.


KEY FEATURES

Fluorescence & brightfield scanning

Extended depth of focus

Compatible with oversized slides

One click-automation

TMA & FISH/CISH

Multi-channel fluorescence & brightfield scanning

Oil objectives

Quantitative image analysis

Live imaging option

Inverted configuration available

High-Speed fluorescence camera option

Full magnification objective scanning (2.5X-100X)

Dot analysis - RNA in situ hybridisation

RNA in situ hybridisation provides information on the spatial expression of RNA in tissue cells, and Dot marker analysis automatically calculates the number of RNA scopes in the nucleus/cytoplasm. In addition, this function is also suitable for dot marker analysis such as FISH.

Left: Identification of the dot fluorescence signal in the TxR channel, i.e. the RNA signal (marked in yellow).

Right figure: TxR channel punctate signals within 5 μm of the cell nucleus are notated to identify RNA positive cells (purple marker); the horizontal coordinate is the number of RNA signal dots in the cell, and the vertical coordinate is the total area of RNA signal dots in the cell RNA-positive cells are identified within the gate.